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a37573 recombinant npc1 protein  (Creative BioMart)


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    Creative BioMart a37573 recombinant npc1 protein
    A37573 Recombinant Npc1 Protein, supplied by Creative BioMart, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/a37573+recombinant+npc1+protein/Recombinant+Human+NPC1+protein%2C+His%2FFLAG-tagged/pmc06104738__mmc2-473-70-75
    Average 88 stars, based on 1 article reviews
    a37573 recombinant npc1 protein - by Bioz Stars, 2026-09
    88/100 stars

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    Recombinant:

    Article Title: Multifunctional Pan-ebolavirus Antibody Recognizes a Site of Broad Vulnerability on the Ebolavirus Glycoprotein
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 4G7/Alexa Fluor 647 This paper N/A BDBV289/Alexa Fluor 647 This paper N/A BDBV317/Alexa Fluor 647 This paper N/A BDBV223/Alexa Fluor 647 This paper N/A EBOV-442/Alexa Fluor 647 This paper N/A PE anti-DYKDDDDK (FLAG) Tag Antibody (clone L5) BioLegend Cat# 637310 Alexa Fluor 488 AffiniPure Goat Anti-Human IgG Jackson ImmunoResearch Laboratories Cat# 109-545-006 2G12 Polymun Scientifics Cat# AB002 c13C6 IBT Bioservices Pacific Blue anti-human CD66b Antibody (clone G10F5) BioLegend Cat# 305112 Alexa Fluor 700 Mouse Anti-Human CD3 (clone UCHT1) BD Biosciences Cat# 557943 APC-Cy7 Mouse Anti-Human CD14 (clone M4P9) BD Biosciences Cat# 561709 PE-Cy5 Mouse Anti-Human CD107a (clone H4A3) BD Biosciences Cat# 555802 PE-Cy7 Mouse Anti-Human CD56 (clone B159) BD Biosciences Cat# 557747 APC-Cy7 Mouse Anti-Human CD16 (clone 3G8) BD Biosciences Cat# 557758 APC Mouse Anti-Human IFN-g (clone B27) BD Biosciences Cat# 554702 PE Mouse Anti-Human MIP-1b (clone D21-1351) BD Biosciences Cat# 550078 Goat-anti rabbit IgG polyclonal antibody/HRP KPL Cat# 474-1516 Bacterial and Virus Strains Mouse-adapted EBOV /Mayinga (EBOV/M.mus-tc/ COD/76/Yambuku-Mayinga) Bray et al., 1998 GenBank: AF499101 EBOV-eGFP/Mayinga Towner et al., 2005 N/A Guinea pig-adapted SUDV/ Boniface (SUDV-GA) Wong et al., 2015 GenBank: KT878488 SUDV strain Gulu Sanchez and Rollin, 2005 GenBank: AY729654 BDBV strain 200706291 Uganda Towner et al., 2008 GenBank: FJ217161 Chimeric EBOV/BDBV-GP Ilinykh et al., 2016 GenBank: KU174137 Chimeric EBOV/SUDV-GP Ilinykh et al., 2016 GenBank: KU174142 rVSV/EBOV-GP/Mayinga Garbutt et al., 2004 N/A E106K EBOV-eGFP, mAb EBOV-520 escape mutant This paper N/A P513L EBOV-eGFP, mAb EBOV-515 escape mutant This paper N/A N514D rVSV/EBOV-GP, mAb EBOV-515 escape mutant This paper N/A L273P EBOV-eGFP, mAb EBOV-442 escape mutant This paper N/A L273P rVSV/EBOV-GP, mAb EBOV-442 escape mutant This paper N/A Biological Samples PBMCs from EVD survivor (2013-2016 EVD epidemic in Nigeria) This paper Donor ID #963 PBMCs from EVD survivor (2014 Boende outbreak in the DRC) This paper UCLADRC ES-43923 Plasma fromEVD survivor (2013-2016 EVD epidemic in Nigeria) This paper Donor ID #963 Normal human plasma American Red Cross N/A Chemicals, Peptides, and Recombinant Proteins Standard guinea pig complement CEDARLANE Labs Cat# CL5000 EBOV GP DTM (aa 1-636; Makona) This paper N/A BDBV GP DTM (aa 1-643; 200706291 Uganda) This paper N/A (Continued on next page) e2 Immunity 49, 363–374.e1–e10, August 21, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER SUDV GP DTM (aa 1-637; Gulu) This paper N/A MARV GP DTM (aa 1-648; Angola 2005) This paper N/A EBOV GPDMuc Murin et al., 2014 N/A EBOV GP DTM IBT Bioservices Cat# 0501-016 Thermolysin Promega Cat# 9PIV400 Immobilized papain ThermoFisher Cat# 20341 Brefeldin A Sigma Aldrich Cat# B7651 GolgiStop BD Biosciences Cat# 554724 Step-Tactin resin QIAGEN Cat# 30002 Alexa Fluor 647 NHS ester ThermoFisher Cat# A37573 Recombinant NPC1 protein, His/FLAG-tagged Creative BioMart Cat# NPC1-1339H FluoSpheres NeutrAvidin-Labeled Microspheres ThermoFisher Cat# F-8776 EZ-Link Sulfo-NHS-LC-LC-Biotin ThermoFisher Cat# 21338 1-Step Ultra TMB-ELISA ThermoFisher Cat# 34029 Freestyle 293 expression medium ThermoFisher Cat# 12338002 ExpiCHO Expression Medium ThermoFisher Cat# A2910001 Fetal Bovine Serum, ultra-low IgG ThermoFisher Cat# 16250078 ClonaCell-HY Medium E Stem Cell Technologies Cat# 03805 ClonaCell-HY Medium A Stem Cell Technologies Cat# 03801 Critical Commercial Assays RosetteSep Human NK Cell Enrichment Cocktail Stem Cell Technologies Cat# 15025 Diagnostic Profile Reagent Rotor Package Abaxis Cat# 500-0038 Deposited Data EBOV-515 Fab complex with EBOV GP DTM This paper EMD-7956 EBOV-520 Fab complex with EBOV GP DTM This paper EMD-7955 Experimental Models: Cell Lines Human: Jurkat, clone E6-1 ATCC ATCC: TIB-152 Human: Jurkat-EBOV GP (Makona) Davis and Ahmed, personal communication N/A Mouse: NIH 3T3-hCD40-hIL21-hBAFF D. Bhattacharya N/A Mouse-human HMAA 2.5 myeloma cell line L. Cavacini N/A Hamster: ExpiCHO-S ThermoFisher Scientific Cat# A29127 Human: FreeStyle 293F ThermoFisher Scientific Cat# R79007 Human: THP-1 monocytes ATCC ATCC: TIB-202 Human: EBOV GPkik-293FS EGFP CCR5-SNAP J. Lewis N/A Monkey: Vero-E6 ATCC ATCC: CRL-1586 Drosophila: Schneider 2 ThermoFisher Scientific Cat# R69007 EBOV-434 hybridoma clone This study N/A EBOV-437 hybridoma clone This study N/A EBOV-442 hybridoma clone This study N/A EBOV-446 hybridoma clone This study N/A EBOV-451 hybridoma clone This study N/A EBOV-502 hybridoma clone This study N/A EBOV-507 hybridoma clone This study N/A EBOV-508 hybridoma clone This study N/A EBOV-510 hybridoma clone This study N/A EBOV-511 hybridoma clone This study N/A EBOV-514 hybridoma clone This study N/A EBOV-515 hybridoma clone This study N/A (Continued on next page) Immunity 49, 363–374.e1–e10, August 21, 2018 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER EBOV-517 hybridoma clone This study N/A EBOV-518 hybridoma clone This study N/A EBOV-520 hybridoma clone This study N/A EBOV-524 hybridoma clone This study N/A Experimental Models: Organisms/Strains Mouse: BALB/cJ The Jackson Laboratory N/A Mouse: 129S6/SvEv-Stat1tm1Rds (STAT1 KO) Taconic Biosciences N/A Guinea pig: Hartley Charles River Laboratories N/A Ferret: Outbred Mustela putorius furo Marshall BioResources N/A Recombinant DNA Plasmid: EBOV GP DTM (aa 1-636; Makona) This paper N/A Plasmid: BDBV GP DTM (aa 1-643; 200706291 Uganda) This paper N/A Plasmid: SUDV GP DTM (aa 1-637; Gulu) This paper N/A Plasmid: MARV GP DTM (aa 1-648; Angola 2005) This paper N/A Plasmid: EBOV-520 rIgG1 heavy chain This paper N/A Plasmid: EBOV-520 light chain This paper N/A Plasmid: EBOV-520 rIgG1-LALA heavy chain This paper N/A Plasmid: EBOV-520 Fab heavy chain This paper N/A Software and Algorithms GraphPad Prism 7.2 GraphPad Software, Inc. https://www.graphpad.com FlowJo version 10 Tree Star Inc. https://www.flowjo.com/solutions/ flowjo/downloads ImMunoGeneTics database Giudicelli and Lefranc, 2011 http://www.imgt.org/ ForeCyt Standard 6.2 (R1) Intellicyt https://intellicyt.com/products/ software/ MATLAB (r2015a) MathWorks, Inc. https://www.mathworks.com/ products/matlab.html DoGpicker Voss et al., 2009 http://emg.nysbc.org/redmine/ projects/software/wiki/DoGpicker Appion Lander et al., 2009 http://emg.nysbc.org/redmine/ projects/appion/wiki/Appion_Home Pymol Schrödinger https://www.pymol.org/2// Other VetScan VS2 Chemistry Analyzer Abaxis N/A iQue Screener Plus flow cytometer Intellicyt N/A BD LSR2 (3-laser) flow cytometer BD Biosciences N/A ECM 2001 Electro Cell Manipulator BTX N/A ÄKTA pure chromatography system GE Healthcare N/A Tecnai Spirit electron microscope with TemCam F416 4k x 4k CCD Zhao et al., 2017 N/A Synergy H1 microplate reader BioTek N/A Synergy 2 microplate reader BioTek N/A EL406 washer dispenser BioTek N/A Biostack microplate stacker BioTek N/A StrepTrap HP GE Healthcare Cat# 28-9075-48 HiTrap Protein G High Performance GE Healthcare Cat# 17-0404-01 HiTrap MabSelect SuRe 5 mL column GE Healthcare Cat# 11-0034-93 Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL ThermoFisher Cat# PI-89883 Superdex 200 Increase 10/300 GL column GE Healthcare N/A e4 Immunity 49, 363–374.e1–e10, August 21, 2018

    Expressing:

    Article Title: Multifunctional Pan-ebolavirus Antibody Recognizes a Site of Broad Vulnerability on the Ebolavirus Glycoprotein
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 4G7/Alexa Fluor 647 This paper N/A BDBV289/Alexa Fluor 647 This paper N/A BDBV317/Alexa Fluor 647 This paper N/A BDBV223/Alexa Fluor 647 This paper N/A EBOV-442/Alexa Fluor 647 This paper N/A PE anti-DYKDDDDK (FLAG) Tag Antibody (clone L5) BioLegend Cat# 637310 Alexa Fluor 488 AffiniPure Goat Anti-Human IgG Jackson ImmunoResearch Laboratories Cat# 109-545-006 2G12 Polymun Scientifics Cat# AB002 c13C6 IBT Bioservices Pacific Blue anti-human CD66b Antibody (clone G10F5) BioLegend Cat# 305112 Alexa Fluor 700 Mouse Anti-Human CD3 (clone UCHT1) BD Biosciences Cat# 557943 APC-Cy7 Mouse Anti-Human CD14 (clone M4P9) BD Biosciences Cat# 561709 PE-Cy5 Mouse Anti-Human CD107a (clone H4A3) BD Biosciences Cat# 555802 PE-Cy7 Mouse Anti-Human CD56 (clone B159) BD Biosciences Cat# 557747 APC-Cy7 Mouse Anti-Human CD16 (clone 3G8) BD Biosciences Cat# 557758 APC Mouse Anti-Human IFN-g (clone B27) BD Biosciences Cat# 554702 PE Mouse Anti-Human MIP-1b (clone D21-1351) BD Biosciences Cat# 550078 Goat-anti rabbit IgG polyclonal antibody/HRP KPL Cat# 474-1516 Bacterial and Virus Strains Mouse-adapted EBOV /Mayinga (EBOV/M.mus-tc/ COD/76/Yambuku-Mayinga) Bray et al., 1998 GenBank: AF499101 EBOV-eGFP/Mayinga Towner et al., 2005 N/A Guinea pig-adapted SUDV/ Boniface (SUDV-GA) Wong et al., 2015 GenBank: KT878488 SUDV strain Gulu Sanchez and Rollin, 2005 GenBank: AY729654 BDBV strain 200706291 Uganda Towner et al., 2008 GenBank: FJ217161 Chimeric EBOV/BDBV-GP Ilinykh et al., 2016 GenBank: KU174137 Chimeric EBOV/SUDV-GP Ilinykh et al., 2016 GenBank: KU174142 rVSV/EBOV-GP/Mayinga Garbutt et al., 2004 N/A E106K EBOV-eGFP, mAb EBOV-520 escape mutant This paper N/A P513L EBOV-eGFP, mAb EBOV-515 escape mutant This paper N/A N514D rVSV/EBOV-GP, mAb EBOV-515 escape mutant This paper N/A L273P EBOV-eGFP, mAb EBOV-442 escape mutant This paper N/A L273P rVSV/EBOV-GP, mAb EBOV-442 escape mutant This paper N/A Biological Samples PBMCs from EVD survivor (2013-2016 EVD epidemic in Nigeria) This paper Donor ID #963 PBMCs from EVD survivor (2014 Boende outbreak in the DRC) This paper UCLADRC ES-43923 Plasma fromEVD survivor (2013-2016 EVD epidemic in Nigeria) This paper Donor ID #963 Normal human plasma American Red Cross N/A Chemicals, Peptides, and Recombinant Proteins Standard guinea pig complement CEDARLANE Labs Cat# CL5000 EBOV GP DTM (aa 1-636; Makona) This paper N/A BDBV GP DTM (aa 1-643; 200706291 Uganda) This paper N/A (Continued on next page) e2 Immunity 49, 363–374.e1–e10, August 21, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER SUDV GP DTM (aa 1-637; Gulu) This paper N/A MARV GP DTM (aa 1-648; Angola 2005) This paper N/A EBOV GPDMuc Murin et al., 2014 N/A EBOV GP DTM IBT Bioservices Cat# 0501-016 Thermolysin Promega Cat# 9PIV400 Immobilized papain ThermoFisher Cat# 20341 Brefeldin A Sigma Aldrich Cat# B7651 GolgiStop BD Biosciences Cat# 554724 Step-Tactin resin QIAGEN Cat# 30002 Alexa Fluor 647 NHS ester ThermoFisher Cat# A37573 Recombinant NPC1 protein, His/FLAG-tagged Creative BioMart Cat# NPC1-1339H FluoSpheres NeutrAvidin-Labeled Microspheres ThermoFisher Cat# F-8776 EZ-Link Sulfo-NHS-LC-LC-Biotin ThermoFisher Cat# 21338 1-Step Ultra TMB-ELISA ThermoFisher Cat# 34029 Freestyle 293 expression medium ThermoFisher Cat# 12338002 ExpiCHO Expression Medium ThermoFisher Cat# A2910001 Fetal Bovine Serum, ultra-low IgG ThermoFisher Cat# 16250078 ClonaCell-HY Medium E Stem Cell Technologies Cat# 03805 ClonaCell-HY Medium A Stem Cell Technologies Cat# 03801 Critical Commercial Assays RosetteSep Human NK Cell Enrichment Cocktail Stem Cell Technologies Cat# 15025 Diagnostic Profile Reagent Rotor Package Abaxis Cat# 500-0038 Deposited Data EBOV-515 Fab complex with EBOV GP DTM This paper EMD-7956 EBOV-520 Fab complex with EBOV GP DTM This paper EMD-7955 Experimental Models: Cell Lines Human: Jurkat, clone E6-1 ATCC ATCC: TIB-152 Human: Jurkat-EBOV GP (Makona) Davis and Ahmed, personal communication N/A Mouse: NIH 3T3-hCD40-hIL21-hBAFF D. Bhattacharya N/A Mouse-human HMAA 2.5 myeloma cell line L. Cavacini N/A Hamster: ExpiCHO-S ThermoFisher Scientific Cat# A29127 Human: FreeStyle 293F ThermoFisher Scientific Cat# R79007 Human: THP-1 monocytes ATCC ATCC: TIB-202 Human: EBOV GPkik-293FS EGFP CCR5-SNAP J. Lewis N/A Monkey: Vero-E6 ATCC ATCC: CRL-1586 Drosophila: Schneider 2 ThermoFisher Scientific Cat# R69007 EBOV-434 hybridoma clone This study N/A EBOV-437 hybridoma clone This study N/A EBOV-442 hybridoma clone This study N/A EBOV-446 hybridoma clone This study N/A EBOV-451 hybridoma clone This study N/A EBOV-502 hybridoma clone This study N/A EBOV-507 hybridoma clone This study N/A EBOV-508 hybridoma clone This study N/A EBOV-510 hybridoma clone This study N/A EBOV-511 hybridoma clone This study N/A EBOV-514 hybridoma clone This study N/A EBOV-515 hybridoma clone This study N/A (Continued on next page) Immunity 49, 363–374.e1–e10, August 21, 2018 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER EBOV-517 hybridoma clone This study N/A EBOV-518 hybridoma clone This study N/A EBOV-520 hybridoma clone This study N/A EBOV-524 hybridoma clone This study N/A Experimental Models: Organisms/Strains Mouse: BALB/cJ The Jackson Laboratory N/A Mouse: 129S6/SvEv-Stat1tm1Rds (STAT1 KO) Taconic Biosciences N/A Guinea pig: Hartley Charles River Laboratories N/A Ferret: Outbred Mustela putorius furo Marshall BioResources N/A Recombinant DNA Plasmid: EBOV GP DTM (aa 1-636; Makona) This paper N/A Plasmid: BDBV GP DTM (aa 1-643; 200706291 Uganda) This paper N/A Plasmid: SUDV GP DTM (aa 1-637; Gulu) This paper N/A Plasmid: MARV GP DTM (aa 1-648; Angola 2005) This paper N/A Plasmid: EBOV-520 rIgG1 heavy chain This paper N/A Plasmid: EBOV-520 light chain This paper N/A Plasmid: EBOV-520 rIgG1-LALA heavy chain This paper N/A Plasmid: EBOV-520 Fab heavy chain This paper N/A Software and Algorithms GraphPad Prism 7.2 GraphPad Software, Inc. https://www.graphpad.com FlowJo version 10 Tree Star Inc. https://www.flowjo.com/solutions/ flowjo/downloads ImMunoGeneTics database Giudicelli and Lefranc, 2011 http://www.imgt.org/ ForeCyt Standard 6.2 (R1) Intellicyt https://intellicyt.com/products/ software/ MATLAB (r2015a) MathWorks, Inc. https://www.mathworks.com/ products/matlab.html DoGpicker Voss et al., 2009 http://emg.nysbc.org/redmine/ projects/software/wiki/DoGpicker Appion Lander et al., 2009 http://emg.nysbc.org/redmine/ projects/appion/wiki/Appion_Home Pymol Schrödinger https://www.pymol.org/2// Other VetScan VS2 Chemistry Analyzer Abaxis N/A iQue Screener Plus flow cytometer Intellicyt N/A BD LSR2 (3-laser) flow cytometer BD Biosciences N/A ECM 2001 Electro Cell Manipulator BTX N/A ÄKTA pure chromatography system GE Healthcare N/A Tecnai Spirit electron microscope with TemCam F416 4k x 4k CCD Zhao et al., 2017 N/A Synergy H1 microplate reader BioTek N/A Synergy 2 microplate reader BioTek N/A EL406 washer dispenser BioTek N/A Biostack microplate stacker BioTek N/A StrepTrap HP GE Healthcare Cat# 28-9075-48 HiTrap Protein G High Performance GE Healthcare Cat# 17-0404-01 HiTrap MabSelect SuRe 5 mL column GE Healthcare Cat# 11-0034-93 Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL ThermoFisher Cat# PI-89883 Superdex 200 Increase 10/300 GL column GE Healthcare N/A e4 Immunity 49, 363–374.e1–e10, August 21, 2018

    Diagnostic Assay:

    Article Title: Multifunctional Pan-ebolavirus Antibody Recognizes a Site of Broad Vulnerability on the Ebolavirus Glycoprotein
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 4G7/Alexa Fluor 647 This paper N/A BDBV289/Alexa Fluor 647 This paper N/A BDBV317/Alexa Fluor 647 This paper N/A BDBV223/Alexa Fluor 647 This paper N/A EBOV-442/Alexa Fluor 647 This paper N/A PE anti-DYKDDDDK (FLAG) Tag Antibody (clone L5) BioLegend Cat# 637310 Alexa Fluor 488 AffiniPure Goat Anti-Human IgG Jackson ImmunoResearch Laboratories Cat# 109-545-006 2G12 Polymun Scientifics Cat# AB002 c13C6 IBT Bioservices Pacific Blue anti-human CD66b Antibody (clone G10F5) BioLegend Cat# 305112 Alexa Fluor 700 Mouse Anti-Human CD3 (clone UCHT1) BD Biosciences Cat# 557943 APC-Cy7 Mouse Anti-Human CD14 (clone M4P9) BD Biosciences Cat# 561709 PE-Cy5 Mouse Anti-Human CD107a (clone H4A3) BD Biosciences Cat# 555802 PE-Cy7 Mouse Anti-Human CD56 (clone B159) BD Biosciences Cat# 557747 APC-Cy7 Mouse Anti-Human CD16 (clone 3G8) BD Biosciences Cat# 557758 APC Mouse Anti-Human IFN-g (clone B27) BD Biosciences Cat# 554702 PE Mouse Anti-Human MIP-1b (clone D21-1351) BD Biosciences Cat# 550078 Goat-anti rabbit IgG polyclonal antibody/HRP KPL Cat# 474-1516 Bacterial and Virus Strains Mouse-adapted EBOV /Mayinga (EBOV/M.mus-tc/ COD/76/Yambuku-Mayinga) Bray et al., 1998 GenBank: AF499101 EBOV-eGFP/Mayinga Towner et al., 2005 N/A Guinea pig-adapted SUDV/ Boniface (SUDV-GA) Wong et al., 2015 GenBank: KT878488 SUDV strain Gulu Sanchez and Rollin, 2005 GenBank: AY729654 BDBV strain 200706291 Uganda Towner et al., 2008 GenBank: FJ217161 Chimeric EBOV/BDBV-GP Ilinykh et al., 2016 GenBank: KU174137 Chimeric EBOV/SUDV-GP Ilinykh et al., 2016 GenBank: KU174142 rVSV/EBOV-GP/Mayinga Garbutt et al., 2004 N/A E106K EBOV-eGFP, mAb EBOV-520 escape mutant This paper N/A P513L EBOV-eGFP, mAb EBOV-515 escape mutant This paper N/A N514D rVSV/EBOV-GP, mAb EBOV-515 escape mutant This paper N/A L273P EBOV-eGFP, mAb EBOV-442 escape mutant This paper N/A L273P rVSV/EBOV-GP, mAb EBOV-442 escape mutant This paper N/A Biological Samples PBMCs from EVD survivor (2013-2016 EVD epidemic in Nigeria) This paper Donor ID #963 PBMCs from EVD survivor (2014 Boende outbreak in the DRC) This paper UCLADRC ES-43923 Plasma fromEVD survivor (2013-2016 EVD epidemic in Nigeria) This paper Donor ID #963 Normal human plasma American Red Cross N/A Chemicals, Peptides, and Recombinant Proteins Standard guinea pig complement CEDARLANE Labs Cat# CL5000 EBOV GP DTM (aa 1-636; Makona) This paper N/A BDBV GP DTM (aa 1-643; 200706291 Uganda) This paper N/A (Continued on next page) e2 Immunity 49, 363–374.e1–e10, August 21, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER SUDV GP DTM (aa 1-637; Gulu) This paper N/A MARV GP DTM (aa 1-648; Angola 2005) This paper N/A EBOV GPDMuc Murin et al., 2014 N/A EBOV GP DTM IBT Bioservices Cat# 0501-016 Thermolysin Promega Cat# 9PIV400 Immobilized papain ThermoFisher Cat# 20341 Brefeldin A Sigma Aldrich Cat# B7651 GolgiStop BD Biosciences Cat# 554724 Step-Tactin resin QIAGEN Cat# 30002 Alexa Fluor 647 NHS ester ThermoFisher Cat# A37573 Recombinant NPC1 protein, His/FLAG-tagged Creative BioMart Cat# NPC1-1339H FluoSpheres NeutrAvidin-Labeled Microspheres ThermoFisher Cat# F-8776 EZ-Link Sulfo-NHS-LC-LC-Biotin ThermoFisher Cat# 21338 1-Step Ultra TMB-ELISA ThermoFisher Cat# 34029 Freestyle 293 expression medium ThermoFisher Cat# 12338002 ExpiCHO Expression Medium ThermoFisher Cat# A2910001 Fetal Bovine Serum, ultra-low IgG ThermoFisher Cat# 16250078 ClonaCell-HY Medium E Stem Cell Technologies Cat# 03805 ClonaCell-HY Medium A Stem Cell Technologies Cat# 03801 Critical Commercial Assays RosetteSep Human NK Cell Enrichment Cocktail Stem Cell Technologies Cat# 15025 Diagnostic Profile Reagent Rotor Package Abaxis Cat# 500-0038 Deposited Data EBOV-515 Fab complex with EBOV GP DTM This paper EMD-7956 EBOV-520 Fab complex with EBOV GP DTM This paper EMD-7955 Experimental Models: Cell Lines Human: Jurkat, clone E6-1 ATCC ATCC: TIB-152 Human: Jurkat-EBOV GP (Makona) Davis and Ahmed, personal communication N/A Mouse: NIH 3T3-hCD40-hIL21-hBAFF D. Bhattacharya N/A Mouse-human HMAA 2.5 myeloma cell line L. Cavacini N/A Hamster: ExpiCHO-S ThermoFisher Scientific Cat# A29127 Human: FreeStyle 293F ThermoFisher Scientific Cat# R79007 Human: THP-1 monocytes ATCC ATCC: TIB-202 Human: EBOV GPkik-293FS EGFP CCR5-SNAP J. Lewis N/A Monkey: Vero-E6 ATCC ATCC: CRL-1586 Drosophila: Schneider 2 ThermoFisher Scientific Cat# R69007 EBOV-434 hybridoma clone This study N/A EBOV-437 hybridoma clone This study N/A EBOV-442 hybridoma clone This study N/A EBOV-446 hybridoma clone This study N/A EBOV-451 hybridoma clone This study N/A EBOV-502 hybridoma clone This study N/A EBOV-507 hybridoma clone This study N/A EBOV-508 hybridoma clone This study N/A EBOV-510 hybridoma clone This study N/A EBOV-511 hybridoma clone This study N/A EBOV-514 hybridoma clone This study N/A EBOV-515 hybridoma clone This study N/A (Continued on next page) Immunity 49, 363–374.e1–e10, August 21, 2018 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER EBOV-517 hybridoma clone This study N/A EBOV-518 hybridoma clone This study N/A EBOV-520 hybridoma clone This study N/A EBOV-524 hybridoma clone This study N/A Experimental Models: Organisms/Strains Mouse: BALB/cJ The Jackson Laboratory N/A Mouse: 129S6/SvEv-Stat1tm1Rds (STAT1 KO) Taconic Biosciences N/A Guinea pig: Hartley Charles River Laboratories N/A Ferret: Outbred Mustela putorius furo Marshall BioResources N/A Recombinant DNA Plasmid: EBOV GP DTM (aa 1-636; Makona) This paper N/A Plasmid: BDBV GP DTM (aa 1-643; 200706291 Uganda) This paper N/A Plasmid: SUDV GP DTM (aa 1-637; Gulu) This paper N/A Plasmid: MARV GP DTM (aa 1-648; Angola 2005) This paper N/A Plasmid: EBOV-520 rIgG1 heavy chain This paper N/A Plasmid: EBOV-520 light chain This paper N/A Plasmid: EBOV-520 rIgG1-LALA heavy chain This paper N/A Plasmid: EBOV-520 Fab heavy chain This paper N/A Software and Algorithms GraphPad Prism 7.2 GraphPad Software, Inc. https://www.graphpad.com FlowJo version 10 Tree Star Inc. https://www.flowjo.com/solutions/ flowjo/downloads ImMunoGeneTics database Giudicelli and Lefranc, 2011 http://www.imgt.org/ ForeCyt Standard 6.2 (R1) Intellicyt https://intellicyt.com/products/ software/ MATLAB (r2015a) MathWorks, Inc. https://www.mathworks.com/ products/matlab.html DoGpicker Voss et al., 2009 http://emg.nysbc.org/redmine/ projects/software/wiki/DoGpicker Appion Lander et al., 2009 http://emg.nysbc.org/redmine/ projects/appion/wiki/Appion_Home Pymol Schrödinger https://www.pymol.org/2// Other VetScan VS2 Chemistry Analyzer Abaxis N/A iQue Screener Plus flow cytometer Intellicyt N/A BD LSR2 (3-laser) flow cytometer BD Biosciences N/A ECM 2001 Electro Cell Manipulator BTX N/A ÄKTA pure chromatography system GE Healthcare N/A Tecnai Spirit electron microscope with TemCam F416 4k x 4k CCD Zhao et al., 2017 N/A Synergy H1 microplate reader BioTek N/A Synergy 2 microplate reader BioTek N/A EL406 washer dispenser BioTek N/A Biostack microplate stacker BioTek N/A StrepTrap HP GE Healthcare Cat# 28-9075-48 HiTrap Protein G High Performance GE Healthcare Cat# 17-0404-01 HiTrap MabSelect SuRe 5 mL column GE Healthcare Cat# 11-0034-93 Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL ThermoFisher Cat# PI-89883 Superdex 200 Increase 10/300 GL column GE Healthcare N/A e4 Immunity 49, 363–374.e1–e10, August 21, 2018



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